Time-lapse imaging of Drosophila testis for monitoring actin dynamics and sperm release
STAR Protocols(2022)
摘要
Here we describe a simple step-by-step protocol for collecting high-resolution, time-lapse images of intact Drosophila testis ex vivo for a limited period using a confocal microscope, with minimum photo-toxic damage, to monitor spermatid individualization, coiling, and release. The F-actin dynamics during spermatid morphogenesis can be further investigated through laser ablations, Fluorescence-Recovery-After-Photobleaching, and drug treatments, using this protocol.
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关键词
Cell Biology,Developmental biology,Microscopy,Model Organisms
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