Investigation of phenolic compounds at the leaves and shoots Arctostaphylos spp. and their antioxidant and antityrosinase activities.

Comparative phytochemical and pharmacological analysis of leaves and shoots of (L.) Spreng. and endemic of Transcaucasia and North Caucasia Lipsch. was carried out. Phenolic compounds in methanol extracts were investigated by HPLC-PDA-ESI-MS. Pharmacological investigation was carried out at the models of generation of superoxide, DPPH, hydroxyl and nitrosyl radicals. The antityrosinase properties were evaluated in the reaction of tyrosinase inhibition using L -tyrosine as a substrate. Five new compounds were identified in the shoots and leaves of : 4,6-di-О-galloylglucose; 2,3-di-О-galloylglucose; 1,4,6-tri-О-galloylglucose; 1,2,4,6-tetra-О-galloylglucose; tetra-О-galloylhexose. Thirty-five phenolic compounds and two saponins were identified for the first time in . Water-alcohol extract (70% ethanol) from had the highest antiradical activity. Water extracts from both species had antityrosinase activity and possessed comparable efficiency (IC = 36.4 ± 1.2 µg/ml and IC = 35,5 ± 2 µg/ml, respectively).