Using Methanol to Preserve Retinas for Immunostaining.

Experimental studies on retinal vasculature and retinal ganglion cells (RGCs) investigating the developmental and pathological conditions of the retina mainly rely on whole‐mount retinal immunostaining. Methanol, an auxiliary fixed medium for retinal whole‐mount preparations, has been used in some studies; however, its application in short‐ and long‐term storage of retinas for further study has not been well described. We aimed to evaluate methanol use as a preservation treatment for further immunostaining of the retina.