Chemical Treatments for Insect Cell Differentiation: The Effects of 20-Hydroxyecdysone and Veratridine on Cultured Spodoptera frugiperda (Sf21) Insect Cell Ultrastructure

Simple Summary Cultured insect Sf21 cells treated with the hormone 20-hydroxyecdysone grow long processes and resemble neurons. They also make physical contact with one another and appear to have the potential to form synapses, areas in which nerve cells are in close contact and communicate with one another electrically or by the release of chemical transmitters. This study uses electron microscopy to look for structural evidence of synapses in 20-hydroxyexdysone treated Sf21 cell cultures. Unfortunately, no evidence of synaptic structures were observed, suggesting that other factors are required for the formation of functional synapses in these cultures. Previous studies have shown that insect cell cultures stop dividing, form clumps, and can be induced to grow processes reminiscent of axons, when the culture medium is supplemented with 20-hydroxyecdysone, insulin, or an agent that mimics their action, such as the ecdysone agonist, methoxyfenozide. Those cell growing processes resemble nerve cells, and the present study evaluates the ultrastructure of these cultures by transmission electron microscopy. Sf21 cells treated with 20-hydroxyecdysone (with or without veratridine amendment) and subjected to ultrastructural analysis had a similar somatic appearance to control cells, with slight changes in organelles and organization, such as a greater number of cytoplasmic vacuoles and mitochondrial granules. Finger-like projections were observed between control and treated cells. However, no structural markers of synaptic contacts (e.g., vesicles or synaptic thickenings) were observed in controls, 20-hydroxyecdysone, or 20-hydroxyecdysone + veratridine treated cells. It is concluded that additional agents would be required to induce functional synaptogenesis in Sf21 cells.