Sneathiella sedimenti sp. nov., isolated from marine sediment

A novel bacterium, designated CAU 1612(T), was isolated from coastal sediment sampled in the Republic of Korea. Cells of the strain were Gram-stain-negative, rod-shaped and motile. Optimum growth occurred at 30 degrees C, at pH 7.0 and with 1.0% (w/v) NaCl. Based on 165 rRNA gene sequences, phylogenetic analysis revealed that strain CAU 1612(T) belonged to the genus Sneathiella and showed highest similarity to 'Sneathiella litorea' JCM 33810(T) (98.5%), followed by Sneathiella chungangensis KCTC 32476(T) (97.7%), Sneathiella aquimaris KCTC 12842(T) (96.0%), Sneathiella chinensis LMG 23452(T) (95.5%), Sneathiella limimaris KCTC 52846(T) (95.5%) and Sneathiella glossodoripedis KCTC 12842(T) (95.4%). The average nucleotide identity and digital DNA-DNA hybridization values of strain CAU 1612(T) were estimated to be 68.5-81.7% and <= 23.7%, respectively, to members of the genus Sneathiella. The DNA G+C content was 54.1 mol%. The major cellular fatty acids (>10%) were C-16(:0), C-1(9:)0 cyclo omega 8c and summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c). The major menaquinone was Q-10 and the predominant polar lipids were phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. On the basis of taxonomic evidence, strain CAU 1612(T) represents a novel species, for which the name Sneathiella sedimenti sp. nov. is proposed. The type strain is CAU 1612(T)(=KCTC 82427(T)=MCCC 1K06082(T)).