Residual DNA in enzymes may pose a challenge for DIY RNA sequencing protocols: how to deal

High-throughput RNA sequencing (RNA-seq) is widely employed for gene expression profiling. This report on do-it-yourself RNA-Seq library preparation pitfalls, highlighting their correct identification and successful overcoming, may be helpful to research groups commencing a DIY RNA-Seq workflow and facing similar problems. ### Competing Interest Statement The authors have declared no competing interest.