L arkinella humicola sp. nov., a gamma radiation-resistant bacterium isolated from soil

A Gram-negative, short rod-shaped, and pink-pigmented bacterial strain, designated MA1 T , was isolated from a soil sample from Gijang-gun, Busan in Republic of Korea. The 16S rRNA gene sequence analysis showed that strain MA1 T belonged to the genus Larkinella and was closely related to “ Larkinella punicea ” (97.5% similarity), Larkinella rosea 15J16-1T3A T (96.5%), and Larkinella knui 15J6-3T6 T (96.2%). Polar lipid profile of strain MA1 T contained phosphatidylethanolamine, two unidentified aminolipids, and three unidentified lipids. Menaquinone-7 was the only quinone and the main fatty acids were C 16:1 ω 5c (36.7%), iso-C 15:0 (30.0%), iso-C 17:0 3-OH (7.7%), and summed feature 3 (C 16:1 ω 6c and/or C 16:1 ω 7c and/or iso-C 15:0 2-OH) (7.3%). The genomic DNA G + C content was 52.3 mol% based on the whole-genome analysis. Strain MA1 T exhibited a relatively low level of ANI and in silico DDH values with “ Larkinella punicea ” (91.9 and 47.1%, respectively), Larkinella rosea (79.7 and 23.3%), and Larkinella knui ( 81.9 and 25.7%). Based on its phenotypic properties and phylogenetic distinctiveness, strain MA1 T should be classified in the genus Larkinella as a representative of a novel species, for which the name Larkinella humicola sp. nov. is proposed. The type strain is MA1 T (= KCTC 72629 T = NBRC 114191 T ).