Phenotypes of Monocytes and Monocyte Derived Dendritic Cells (MODC) in Antiretroviral Naïve Chronically Infected HIV Patients

AIDS Research and Human RetrovirusesVol. 30, No. S1 Innate ImmunityFree AccessPhenotypes of Monocytes and Monocyte Derived Dendritic Cells (MODC) in Antiretroviral Naïve Chronically Infected HIV PatientsNadesh Ngechae Nji, Jules C. Tchadji, Achile Nangue Nangue, Georgia Ambada, Carol Stephanie Ngane Sake, Abel Lissom, Edith Temgoua, Betrand Sagnia, Samuel Sosso, Rachel Kamgaing, Jule Clement Assob Nguedia, and Godwin Wapimawah NchindaNadesh Ngechae NjiChantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Microbiology and Immunology Laboratory, Yaounde, CameroonSearch for more papers by this author, Jules C. TchadjiChantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Microbiology and Immunology Laboratory, Yaounde, CameroonSearch for more papers by this author, Achile Nangue NangueChantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Microbiology and Immunology Laboratory, Yaounde, CameroonSearch for more papers by this author, Georgia AmbadaChantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Microbiology and Immunology Laboratory, Yaounde, CameroonSearch for more papers by this author, Carol Stephanie Ngane SakeChantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Microbiology and Immunology Laboratory, Yaounde, CameroonSearch for more papers by this author, Abel LissomChantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Microbiology and Immunology Laboratory, Yaounde, CameroonSearch for more papers by this author, Edith TemgouaChantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Infirmary, Yaounde, CameroonSearch for more papers by this author, Betrand SagniaChantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Microbiology and Immunology Laboratory, Yaounde, CameroonSearch for more papers by this author, Samuel SossoChantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Medical Analysis Laboratory, Yaounde, CameroonSearch for more papers by this author, Rachel KamgaingChantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Medical Analysis Laboratory, Yaounde, CameroonSearch for more papers by this author, Jule Clement Assob NguediaFaculty of Health Sciences, University of Buea, Medcine, Buea, CameroonSearch for more papers by this author, and Godwin Wapimawah NchindaChantal Biya International Reference Center for Research on the Prevention and Management of HIV/AIDS, Microbiology and Immunology Laboratory, Yaounde, CameroonSearch for more papers by this authorPublished Online:30 Oct 2014https://doi.org/10.1089/aid.2014.5250.abstractAboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail P12.11Background: Monocytes are immune cells which differentiate into antigen presenting cells such as dendritic cells (DC). Monocytes have been sub divided into three subclasses, based on CD14 and CD16 surface markers; namely classical (CD14++CD16+), intermediate (CD14++CD16+) and non-classical (CD14+CD16+, CD14+CD16+) monocytes. There is a variation in the phenotypes of these subpopulations in HIV-1 chronically. Monocytes, are used to obtain MoDC in vitro, and in vivo for several immunological studies. It is thus imperative to verify if the variation between the different subclasses of monocytes can impact their ability to be converted to MoDCs.Methods: PBMCs purified from peripheral blood of participants, were used for surface staining with fluorochrome-conjugated antibodies characteristic of monocytes; and also to enrich for monocytes using CD14 magnetic beads and magnetic separation columns. The enriched monocytes were cultured in MoDC differentiation medium to obtain immature MoDCs, in which maturation signal was added (polyICLC) and cultured. Surface staining using antibodies was done at each stage to determine the phenotypic characteristics of the cell populations.Results: A fourth class of monocytes based on their expression of CD14 and CD16, (CD14++CD16+), which had a direct correlation with CD4+ absolute counts and an indirect correlation with viral load was noticed. There was progressive down regulation of CD14 on the monocytes as they converted to MoDCs and up regulation of maturation markers of DCs (CD80, CD86, CD11c, CD1a and CD83).Conclusions: Despite a variation in the different monocyte populations observed between HIV infected people and HIV negative donors, the monocytes still convert to MoDC similarly.FiguresReferencesRelatedDetails Volume 30Issue S1Oct 2014 InformationCopyright 2014, Mary Ann Liebert, Inc.To cite this article:Nadesh Ngechae Nji, Jules C. Tchadji, Achile Nangue Nangue, Georgia Ambada, Carol Stephanie Ngane Sake, Abel Lissom, Edith Temgoua, Betrand Sagnia, Samuel Sosso, Rachel Kamgaing, Jule Clement Assob Nguedia, and Godwin Wapimawah Nchinda.Phenotypes of Monocytes and Monocyte Derived Dendritic Cells (MODC) in Antiretroviral Naïve Chronically Infected HIV Patients.AIDS Research and Human Retroviruses.Oct 2014.A127-A128.http://doi.org/10.1089/aid.2014.5250.abstractPublished in Volume: 30 Issue S1: October 30, 2014PDF download