Integrative Extraction of Primary Metabolites and Proteins from Deinococcus Radiodurans V1

This protocol describes a procedure how primary metabolites and proteins can be extracted from Deinococcus radiodurans simultaneously. Polar metabolites are purified and derivatized for GC-MS (TOF) analysis. Proteins are tryptically digested in-gel and peptides are prepared for bottom-up proteomics by LC-MS (orbitrap).