Statistics of Curve Fitting

In many cases, the goal of ITC measurements is to infer quantitative values for reaction parameters such as equilibrium constants, enthalpy changes, and rate constants. To do this, a mathematical model of the reaction(s) going on in the calorimeter is first constructed. Then the predictions of the model are compared against the experimental observations. Because the model includes several unknown parameters, it is necessary to vary the parameters until the model predictions are “as close as possible” to the observed data.