Multiphoton Imaging of Surgical Pathology

Fluorescent imaging combined with H&E-rendering algorithms can provide histological imaging of surgical specimens more rapidly than conventional histology by avoiding the need to formalin fix, freeze or physically section specimens. However, validation of histology performed on live tissue is challenging because fixation and sectioning frequently result in changes to tissue appearance, while histological cutting planes are often substantially different than the true tissue surface. We present validation studies aiming to understand the relationship between features visualized using multiphoton fluorescence and conventional histology.