The structure ofINI1/hSNF5RPT1 and its interactions with the c‐MYC:MAXheterodimer provide insights into the interplay betweenMYCand theSWI/SNFchromatin remodeling complex

c‐ MYC and the SWI / SNF chromatin remodeling complex act as master regulators of transcription, and play a key role in human cancer. Although they are known to interact, the molecular details of their interaction are lacking. We have determined the structure of the RPT 1 region of the INI 1/ hSNF 5/ BAF 47/ SMARCB 1 subunit of the SWI / SNF complex that acts as a c‐ MYC ‐binding domain, and have localized the interaction regions on both INI 1 and on the c‐ MYC : MAX heterodimer. c‐ MYC interacts with a highly conserved groove on INI 1, while INI 1 binds to the c‐ MYC helix‐loop‐helix region. The binding site overlaps with the c‐ MYC DNA ‐binding region, and we show that binding of INI 1 and E‐box DNA to c‐ MYC : MAX are mutually exclusive.