Physiology and Transcriptomic analysis of Saccharomyces cerevisiae in the presence of inhibitors derived from lignocellulosic biomass

Production of biofuels from lignocellulosic (LC) residues should be fuelingthe energy matrix in the near future. Yeasts will play an important role as platformmicroorganisms for the conversion processes of LC-derived sugars into, for instance,fuel ethanol but also other advanced fuels and chemicals. Fermentation of LChydrolysates poses many scientific and technological challenges. For example, thepretreatment processes generates various yeast growth inhibitors (furan-derivatives,phenolics and organic acids), reducing the efficiency of fermentation. Therefore, weaimed to investigate the effect of selected inhibitory compounds on the quantitativephysiology of yeasts as well as on their transcriptional responses. For that, two S.cerevisiae strains were chosen: the lab strain S. cerevisiae CEN.PK113-7D, a referencelaboratory strain for physiological studies and functional genomics, and the robustindustrial strain S. cerevisiae SA-1, a strain highly tolerant toward inhibitorycompounds and rarely studied so far. The dataset presented so far, indicates importantphysiological differences of industrial strains, such as S. cerevisiae SA-1, in relation tolaboratory ones. Omics data in the presence of inhibitors will contribute further toevaluate these differences.