P4-509: suppression of neuroinflammation and amyloid pathology in 5xfad mice brain by glia maturation factor antibody

Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by accumulation of beta-amyloid (Aβ) plaques and neurofibrillary tangles (NFTs) in the brain. Glia maturation factor (GMF), a neuroinflammatory protein is isolated and cloned in our laboratory play an important role in the pathogenesis of AD. To study the relationship between expression of GMF, glial activation and neuroinflammtion, we investigated the effect of anti-GMF antibody on neuroinflammation, Aβ load and cognitive impairments in the brain of 9- month old 5XFAD mice. Following four weeks of anti-GMF antibody delivery, we observed reduced levels of astrocytic glial fibrillary acidic protein, microglial ionizing calcium binding adaptor molecule 1 and Aβ level in the cerebral cortex of 5XFAD mice brain. Our current study demonstrates that the anti-GMF antibody reduces neuroinflammation and attenuates neurodegeneration in the cortex region of 5XFAD mouse brain. Furthermore, our data suggest that the neutralization of GMF function is a promising strategy to target neuroinflammation/neurodegeneration in AD.