A new network containing MYB109-ZmCesA5 is involved in kernel development
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS(2022)
摘要
MYB genes regulate several different aspects of metabolism and development. However, few studies have reported the involvement of MYBs-CesAs network in the regulation of maize kernel development. In this study, yeast one-hybrid (Y1H) assays and dual-luciferase reporter assays showed that ZmMYB109 activated the expression of ZmCesA5 by directly binding to its promoter. Real-time quantitative PCR (RTqPCR) and transcriptome analyses showed that ZmMYB109 expression increased in ZmCesA5-OE kernels and decreased in ZmCesA5-KO kernels. Overexpression of ZmCesA5 produced heavier kernels, whereas loss of function of ZmCesA5 affected starch and sucrose metabolism, resulting in weight reduction of the maize kernels. Collectively, these findings suggest that a new network containing MYB109-ZmCesA5 is involved in kernel development. (C) 2022 Elsevier Inc. All rights reserved.
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关键词
Maize, Kernel development, ZmCesA5, ZmMYB109
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