Efficient Biodiesel Production Catalysed by Lipase Immobilized on Nanowire Coils

Abstract The lipase from Candida rugosa (CRL) was covalently immobilized on various carbon nanomaterials (functionalized nanowire coils and multi-walled carbon nanotubes) and tested for biodiesel production. Using the most active lipase preparation (covalently immobilized CRL on Nanowire coils) under optimal conditions, 96.8% conversion of waste oil was obtained after only 12 h reaction time. Moreover, the biocatalyst maintained nearly 90% of its initial activity in the batch system after 13 recycling experiments and still 80 % initial activity after storage 240 d.