The involvement of wheat U-box E3 ubiquitin ligase TaPUB1 in salt stress tolerance

U-box E3 ubiquitin ligases play important roles in the ubiquitin/26S proteasome machinery and in abiotic stress responses.TaPUB1-overexpressing wheat (Triticum aestivum L.) were generated to evaluate its function in salt tolerance.These plants had more salt stress tolerance during seedling and flowering stages,whereas the TaPUB1-RNA interference (RNAi)-mediated knock-down transgenic wheat showed more salt stress sensitivity than the wild type (WVT).TaPUB1 overexpression upregulated the expression of genes related to ion channels and increased the net root Na+ efflux,but decreased the net K+ efflux and H+ influx,thereby maintaining a low cytosolic Na+/K+ ratio,compared with the WT.However,RNAi-mediated knock-down plants showed the opposite response to salt stress.TaPUB1 could induce the expression of some genes that improved the antioxidant capacity of plants under salt stress.TaPUB1 also interacted with TaMP (Triticum aestivum α-mannosidase protein),a regulator playing an important role in salt response in yeast and in plants.Thus,low cytosolic Na+/K+ ratios and better antioxidant enzyme activities could be maintained in wheat with overexpression of TaPUB1under salt stress.Therefore,we conclude that the U-box E3 ubiquitin ligase TaPUB1 positively regulates salt stress tolerance in wheat.