From 2D To 3D, Advances of Technology and Instrumentation for Stem Cell Culture in Vitro

Stem cell therapy is a strategic and prospective scientific issue in the international arena. It is of great significance for heart failure, neurodegenerative diseases and many other serious diseases that lack of effective treatments. Large-scale, high-quality, safe and stable stem cell culture technology is a technical bottleneck that restricts the promotion of stem cell therapy. Three-dimensional (3D) culture technology and instruments can significantly improve the production efficiency of stem cells culturing, ensure the production quality, and enhance the therapeutic effects, therefore serves as a foundation for the development of cell therapy filed. In this review, we summarized the working principle of contemporary mainstream 3D stem cell culture instruments as well as their advantages and disadvantages. The future development trend of stem cell culture instrumentation is predicted. Recommendations are proposed for the selection of culturing instruments in stem cell clinical application, and research and development of new technology and instruments in the future.