The Effect of L-Amino Acid Oxidase on Hep-2 Squamous Cell Carcinoma Cell Line

Introduction Head and neck squamous cell carcinoma HNSCC is responsible for more than of head and neck cancers L amino acid oxidases LAAOs are extensively distributed in the following families of venomous snakes the Viperidae Crotalidae and Elapidae families A number of proteins related to LAAO activity have been found to manifest anti neoplastic antimicrobial or apoptosis inducing activity It can be assumed that that the general mechanism of LAOO toxicity is determined by the generation of cytotoxic H O in varying amounts In short the aim of this study is to ascertain the anticancer effect by way of flowcytometry and real time PCR of LAAO on the HEP cell line after hours hours and hours Materials and methods Once theHEP cell line was propagated and LAAO was added to the culture medium the cells were analyzed after hours hours and hours Results The results of this analysis reveal that LAAO was the root cause of apoptosis in the HEP cell line In addition the apoptosis percentage was directly related to time The p expression increased by time and the inverse was true for bcl expression Conclusion The results reveal that LAAO has a substantial effect on the anticancer activity of the HEP cell line