The Affect Of Exercise On Hypothalamic Kiss-1 And Gpr54 In The Puberty Development Of Male Rats: 1936 Board #197 May 31 2 00 PM - 3 30 PM

Kisspeptin, in addition to reflects its ability to inhibit cancer metastasis, which have been implicated in the regulation of GnRH secretion with its receptor GPR54, play an essential role in normal reproduction and pubertal development. PURPOSE: To explore the moderate-intensity treadmill training (MIT) in modulating the hypothalamic expression of kiss-1 and its G protein coupled receptor -GPR54 mRNA relative expression in the different time of puberty. METHODS: 3 weeks male Sprague-Dawley rats (weight:62.03±3.13) were randomly assigned to two experimental groups (n=36): control group (n=18) and the MIT group (n=18). MIT group did the 60%-70%V(·)O2max treadmill training (5 days/week,1 hour/day). All the rats were used to detect the hypothalamic expressions of kiss-1 and GPR54 mRNA, animals in the both groups were sacrificed , which on the week of 5 weeks, 6 weeks, and 8 weeks. RESULT: Compared with C group, every weeks of the rats’ MIT group had lower hypothalamic expression of kiss-1 mRNA and GPR54 mRNA. Especially the kiss-1 mRNA, After moderate-intensity treadmill training, compare with the control groups , the 5 weeks(0.22±0.09 vs 2.28±0.49, p<0.01), 6 weeks(1.09±0.13 vs 1.73±0.18,p<0.01), 8 weeks(0.69±0.21 vs 2.82±0.79) kiss-1 mRNA had significant reduced. All the MIT groups’ hypothalamic expression of GPR54 mRNA had lower than C groups, especially at the age of 6 weeks(0.58±0.1 vs 0.79±0.23,p<0.05). CONCLUSIONS:MIT could reduce the hypothalamic expression of kiss-1 and its receptor GPR54mRNA in the puberty development of male rats, and kiss-1mRNA are more affected.