PO-405 Repositioning existing drugs as novel therapeutics for high-risk paediatric leukaemia

ABSTRACT Introduction Despite remarkable improvements made in the treatment of childhood acute lymphoblastic leukaemia (ALL), prognosis remains dismal for a certain subgroups of high-risk (HR) patients including infants with leukaemia harbouring rearrangement of the Mixed Lineage Leukaemia (MLL/KMT2A) gene. Development of more effective, less toxic therapeutics is therefore urgently needed. The aim of this study was to identify compounds that target HR leukaemia cells based on drug-repurposing, whereby an approved drug is applied to treat a disease other than the one for which it was originally intended. Material and methods New AbstractSelect Abstract A phenotypic screen was performed against HR leukaemia cell lines with a tailored compound library of 3707 approved drugs and pharmacologically active compounds. Results and discussions The screen identified that two FDA-approved drugs, auranofin and disulfiram, originally developed for treatment of rheumatoid arthritis and chronic alcoholism respectively, had preferential cytotoxicity against leukaemia cell lines compared to solid tumours and normal cells (p Conclusion In summary, we have identified two FDA-approved drugs that demonstrated potent, synergistic anti-leukaemia activity through ROS induction as well as chemosensitise cells that are resistant to current chemotherapeutics, potentially opening up new avenues for clinical treatment of HR paediatric leukaemia. We will be testing these therapies in vivo using relevant PDX models of HR paediatric ALL.