Effects of Quercetin on Myocardial Ischemia Reperfusion Injury in Rats

Objective:To investigate the protective mechanism of quercetin(Que) on myocardial ischemia reperfusion injury(MIRI) in rats.Method:The rats were randomly divided control group,MIRI model group,positive control group(diltiazem HCl 0.01 mg · kg-1) and low,medium and high dose Que pretreatment group(15,30,60 mg · kg-1),10 rats each group.The myocardial ischemia reperfusion model was induced by occulusion of the anterior descending coronary artery for 30 min and reperfusion for 60 min.Each Que dose was proconditioned for a week,and the rat's serum and cardiac muscle tissue werecollected seperately 60 minutes after MIRI.The contents of malondialdehyde(MDA),interleukin 6(IL-6),tumor necrosis factor-α(TNF-α),creative kinase MB(CK-MB) and troponin(cTnI);the acitivity of superoxygen dehydrogenises(SOD),glutathione peroxidase(GSH-Px) and Sarcoplasmic reticulum Ca2+ pump(SERCA).And by tetrazolium chloride(TTC) staining,the areas of myocardial damage and infraction were observed.Result:In the MIRI group,the contents of MDA(17.71±3.24)μmol · L-1,CK-MB(2 010.21±76.31) U · L-1,cTnI(2.25±0.76) μg ·L-1,IL-6(5.89±0.34) ng · L-1 and TNF-α(114.12±22.13) μg · L-1 in serum were obviously improved(P0.01 or P0.05),and acitivity of SOD(77.58±5.20) U · mL-1,GSH-Px(11.22±4.88) U · mL-1,and myocardial SERCA(2.76±0.49) μmol · mg-1 · h-1 were obviously reduced(P0.01 or P0.05).The contents of MDA,CK-MB,cTnI,IL-6 and TNF-α in serum in MIRI group were obviously reduced by medium and high dose Que pretreatment(P0.01 or P0.05),and acitivity of SOD,GSH-Px and myocardial SERCA were improved by medium and high dose Que pretreatment(P0.01 or P0.05),and the areas of myocardial damage and infraction were obviously reduced(P0.01 or P0.05).Conclusion:Que preconditioning can alleviate the oxidative stress injury,reduce the inflammatory response and calcium overload in cells,and can indicate obvious protective mechanism on myocardial ischemia/reperfusion injury of rats.