Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity.
STAR protocols(2022)
摘要
Organoids can enable the study of solid tumors initiated from a single cancer stem cell (CSC) vivo. We describe a serial tumor organoid plating protocol using primary colorectal cancer (CRC) tissues as a rapid and cost-efficient approach to evaluate the impact of therapeutic interventions on CSC functions. We detail the isolation of primary colorectal CSCs, organoid embedding, serial passaging, and CSC-related analytical techniques. For complete details on the use and execution of this protocol, please refer to Masibag et al. (2021) and Bergin et al. (2021).
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关键词
Cancer,Cell isolation,Cell-based Assays,Health Sciences,Microscopy,Organoids,Single Cell,Stem Cells,Tissue Engineering
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