Novel Protocols for the Production of Autologous Blood Concentrates With High Platelet Volume.

Autologous blood concentrates (ABCs), specifically platelet-rich plasma (PRP), were originally discussed in the literature more than 60 years ago. Since then, protocols to fabricate PRP from patient-derived blood have steadily evolved with the aim of creating better products with more effective clinical results. Key to the protocol evolution has been the development of ABCs with higher concentrates of platelets and other blood components that enhance soft- and hard-tissue healing and, hence, regeneration. The authors present a protocol for obtaining high-platelet-concentration PRP and autogenous thrombin. This fifth-generation protocol allows PRP to be attained with a platelet concentration six times higher than that which is present in the original clot. This double-centrifugation PRP protocol is relatively simple and inexpensive, as it does not require special equipment or advanced technical knowledge by the practitioner and can readily be conducted in a private dental clinical setting.