Wharton’s Jelly–Mesenchymal Stem Cell–Engineered Conduit for Pulmonary Artery Reconstruction in Growing Piglets

•Tissue engineering technologies are a promising tool to overcome the limitations of currently available materials for RVOT surgery in the pediatric population.•Porcine SIS seeded with allogeneic WJ-MSCs with our technique is a safe and reliable biomaterial for RVOT surgical reconstruction on a growing swine model.•Cell-seeded porcine SIS shows better integration with the host compared to porcine SIS alone, both in terms of histological/microscopic findings and in terms of clinically measurable radiological outcomes, as assessed by echocardiography and cardiovascular magnetic resonance.