Rv1915 and Rv1916 from Mycobacterium tuberculosis H37Rv form in vitro protein-protein complex

•Rv1915 and Rv1916 of Mtb H37Rv associate together to form in vitro protein-protein complex.•Deletion of disordered C-terminal of Rv1915 does not abrogate complex formation with Rv1916.•Site-directed mutagenesis studies show Y64 and Y65 residues of Rv1916 to be crucial for complex formation.•The presence of acetyl-CoA increases the ICL and MICL activities of Rv1916 and the complex.