Two-dimensional Mesoporous Nitrogen-Rich Carbon Nanosheets Loaded with CeO2 Nanoclusters As Nanozymes for the Electrochemical Detection of Superoxide Anions in HepG2 Cells

Two-dimensional (2D) porous carbon-based composite nanosheets loaded with metal oxide nanoclusters are expected to be promising electrocatalysts for high-performance electrochemical sensors. However, for this complicated composite material, strict reaction conditions and complex synthesis steps limit its general application in electrochemical detection. Here we present a facile method to fabricate 2D mesoporous nitrogen-rich carbon nanosheets loaded with CeO2 nanoclusters (2D-mNC@CeO2), for fabricating superoxide anions (O-2(center dot)) electrochemical sensor. The method is based on block copolymers self-assembly and the affinity of polydopamine to metal ions to obtain organic-inorganic hybrid, which can be directly converted into 2D-mNC@CeO2 through carbonization strategy without structural deterioration. Characterizations demonstrate that the 2D-mNC@CeO2 owned the 2D N-doped carbon structure with an interlinked hierarchical mesoporous and the uniformly dispersed CeO2 nanoclusters on the surface. Benefitted from the unique structure, the 2D-mNC@CeO2 shortens electron transfer distance, enhances mass transfer efficiency, exposes numerous active sites, and obtain a high Ce3+/Ce4+ ratio for improving electrocatalytic performance. The 2D-mNC@CeO2/SPCEs sensors for O-2(center dot) detection has a detection limit of 0.179 mu M (S/N = 3) and sensitivity of 401.4 mu A cm(-2) mM-1. The sensors can be applied to capture electrochemical signals of O-2(center dot) released from HepG(2) cells, demonstrating the application potential of the sensors to monitor O-2(center dot) in biological fields.