Single-cell RNA sequencing identifies a migratory keratinocyte subpopulation expressing THBS1 in epidermal wound healing
ISCIENCE(2022)
摘要
Keratinocyte differentiation is an intricate process that is regulated by multiple mediators. Using cultured human keratinocytes, we found that lysophosphatidic acid (LPA) induced the differentiation of a previously unsuspected keratinocyte subpopulation expressing the extracellular matrix protein, thrombospondin-1 (THBS1). This action of LPA was mediated by the RHO/ROCK-SRF signaling down stream of LPA(1) and LPA(5) receptors and required ERK activity. Suppression ofTHBS1 in vitro suggested a migratory role of THBS1(+) keratinocytes. Moreover,we analyzed publicly deposited single-cell RNA sequencing dataset and identified Thbs1-expressing keratinocytes in the mouse wound skin. Immunohistochemistry analysis revealed that Thbs1(+) keratinocytes were apparently differentiated from basal keratinocytes upon wounding, subsequently polarized and migrated suprabasally toward the wound front, and eventually underwent terminal differentiation in the neo-epidermis. Importantly, inhibition of Erk activity suppressed Thbs1(+) keratinocyte differentiation in wound healing.Based on these findings, we suggest that THBS1(+) keratinocyte is a migratory keratinocyte subpopulation that facilitates epidermal wound healing.
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关键词
Biological sciences,Cell biology,Stem cells research
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