Pusillimonas faecipullorum sp. nov., isolated from the poultry manure

A polyphasic taxonomic approach was used to characterize a novel bacterium, designated strain CC-YST705 T , isolated from poultry manure sampled in Taiwan. Cells of strain CC-YST705 T were aerobic, Gram-stain-negative, non-motile, non-spore-forming rods, displaying positive reactions for catalase, oxidase and β -glucosidase. Optimal growth occurred at 30 °C and pH 8. Strain CC-YST705 T shared the highest (> 96.0%) pair-wise 16S rRNA gene sequence similarity with Pusillimonas noertemannii (96.7%), followed by Pusillimonas caeni (96.6%), Eoetvoesia caeni (96.1%) and Paracandidimonas soli (96.0%), and formed a distinct phyletic lineage associate with the clade that accommodated Pusillimonas species. The draft genome (3.1 Mb) having 57.4 mol % G + C content contained genes involved in the catabolism of aromatic hydrocarbons. The orthologous average nucleotide identity (OrthoANI) values were 62.8–73.1% ( n = 8), 72.6 ( n = 1), 71.5% ( n = 1) compared within the type strains of the genera Pusillimonas , Eoetvoesia and Paracandidimonas , respectively. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid, two unidentified phospholipids and five unidentified lipids. The major polyamine was spermidine. The dominating cellular fatty acids (> 5%) included C 12:0 , C 16:0 , C 17:0 cyclo , C 19:0 cyclo ω 8c and 2 C 14:0 3OH/ iso -C 16:1 I. Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence, OrthoANI, digital DDH, and the phylogenomic placement, strain CC-YST705 T is considered to represent a novel species of the genus Pusillimonas , for which the name Pusillimonas faecipullorum sp. nov. is proposed. The type strain is CC-YST705 T (= BCRC 81285 T = JCM 34168 T ).