Assessment of genetic diversity of ninety-one bottle gourd [Lagenaria siceraria (Mol.) Standl.] genotypes from fourteen different agro-climatic zones of India using agro-morphological traits and SSR markers.

BACKGROUND:The evaluation of the magnitude of genetic diversity present in the germplasm collection is prerequisite for bottle gourd improvement programme. The characterization of the diversity pattern of Indian bottle gourd accessions will facilitate the optimal use of genetic resources for breeding improved cultivars. METHODS:In the present study, the magnitude of genetic diversity was evaluated in ninety-one genotypes of bottle gourd collected across fourteen different agro-climatic zones of India. RESULTS:Significant variations were observed for all the studied ten quantitative and nine qualitative traits. The ninety-one genotypes were grouped into nine clusters based on cluster analysis of morphological characteristics. Eigen value from principal component analysis depicted first seven quantitative traits accounted for more than 97.5 cumulative percent of the total variations. The first two components accounted for 50 cumulative percent of the total variation, which signifies a high degree of correlation between the analyzed traits. Molecular diversity with the 40 SSR markers screened revealed 11 polymorphic markers in the genotypes studied. Population structure analysis divulged five populations, conforming to the Principal Coordinate Analysis. Molecular analysis revealed genetically diverse genotypes along with the morphologically divergent genotypes from the quantitative traits and highest inter-cluster distance would be the most appropriate parents for exploiting heterosis. CONCLUSIONS:The results of this study will facilitate the optimal use of genetic resources for breeding improved cultivars of bottle gourd and the adoption of the identified superior genotypes directly by the breeders.