Biotechnological use of dairy by-products for the production and microencapsulation of the food preservative enterocin CRL35

Bacteriocins from Gram-positive bacteria have been proposed as natural food preservative and there is a need for large-scale production for commercial purposes. The aim of the present work is to evaluate whey, a cheese industrial by-product, for the production and microencapsulation of enterocin CRL35. Whey proved to be a promising basal medium for bacterial growth although the bacteriocin production was quite low. However, it could be much favored with the addition of yeast extract at concentrations as low as 0.5%. Besides improving bacteriocin production, this peptide was successfully microencapsulated by spray drying using whey protein concentrate and a chitosan derivative as wall materials. Microcapsules averaging 10 +/- 5 mu m diameter were obtained, with good structural integrity and high antimicrobial activity with a stability of at least 12 weeks at 4 degrees C. In summary, sustainable bacteriocin production and microencapsulation was achieved recycling whey or its derivatives. In addition, the formulation owns high antimicrobial activity with a long shelf life. The development of a food preservative may represent a green solution for handling whey. The production of enterocin CRL35 in whey and yeast extract followed by microencapsulation represents a green solution for industrial byproducts, obtaining an efficient food preservative with long shelf life.