Limiting Dilution Tumor Initiation Assay: An In Vivo Approach for the Study of Cancer Stem Cells.
Methods in molecular biology (Clifton, N.J.)(2022)
摘要
Cancer stem cells (CSCs) are a small subpopulation of self-renewing cancer cells that are present within tumors. Calculating the frequency of tumor-initiating cells is important in the assessment of the number of CSCs present in a cell population. In this chapter, we present a protocol developed for quantification of CSCs from breast cancer tumors that can be adapted to CSCs from other types of tumors.
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关键词
Cancer stem cells,In vivo,Limiting dilution tumor initiation,Tumor-initiating cell frequency
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