Mg 2 +-dependent conformational equilibria in CorA and an 1 integrated view on transport regulation 2

Nicolai Tidemand Johansen* ,1 , Marta Bonaccorsi* ,2 , Tone Bengtsen* ,3,4 , Andreas Haahr 3 Larsen 1,4 , Frederik Grønbæk Tidemand 1 , Martin Cramer Pedersen 1 , Pie Huda 5 , Jens 4 Berndtsson 6 , Tamim Darwish 7 , Nageshwar Rao Yepuri 7 , Anne Martel 8 , Thomas Günther 5 Pomorski 9,10 , Andrea Bertarello 2 , Mark Sansom 4 , Mikaela Rapp 6 , Ramon Crehuet 3 , Tobias 6 Schubeis ,2 , Kresten Lindorff-Larsen ,3 , Guido Pintacuda ,2 , and Lise Arleth ,1 7 8 *Equally contributing authors 9  Corresponding author 10 Emails: tobias.schubeis@ens-lyon.fr, lindorff@bio.ku.dk, guido.pintacuda@ens-lyon.fr, 11 arleth@nbi.ku.dk 12 13 1 Condensed Matter Physics, Niels Bohr Institute, University of Copenhagen, Copenhagen, 14 Denmark 15 2 Centre de RMN à Très hauts Champs de Lyon (UMR 5280, CNRS / Ecole Normale 16 Supérieure de Lyon / Université Claude Bernard Lyon 1), University of Lyon, Villeurbanne, 17 France 18 3 Structural Biology and NMR Laboratory and Linderstrøm-Lang Centre for Protein Science, 19 Department of Biology, University of Copenhagen, Denmark 20 4 Department of Biochemistry, University of Oxford, Oxford, United Kingdom 21 5 Australian Institute for Bioengineering and Nanotechnology, The University of 22 Queensland, Brisbane, Australia 23 6 Department of Biochemistry and Biophysics, Center for Biomembrane Research, 24 Stockholm University, Stockholm, Sweden 25 7 National Deuteration Facility, Australian Nuclear Science and Technology Organization, 26 Lucas Heights, Australia 27 8 Institut Laue–Langevin, Grenoble, France 28 9 Section for Transport Biology, Department of Plant and Environmental Sciences, 29 University of Copenhagen, Frederiksberg, Denmark 30 10 Department of Molecular Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr 31 University Bochum, Germany 32