Evaluation of silver nanoparticles attached to methylene blue as an antimicrobial agent and its cytotoxicity

•Silver nanoparticles were synthesized using different laser power and wavelength.•The Ag NPs were characterized using transmission electron microscopy (TEM), UV-Visible (UV-Vis), and photoluminescence (PL) spectra.•Ag NPs with methylene blue combination were used against gram positive and negative bacteria.•Ag NPs/MB were shown to be more effective than MB and Ag NPs alone.•Ag NPs do not exhibit cytotoxicity up to 50 µg/ml in each solution.