Novel Polyanionic Tracts Improve Expression of a TPP1:K 16 ApoE Chimera.

Alteration of the a priori (GD ) PAT sequence by varying the aspartate and glutamate content was sufficient to prevent cleavage of the resulting TPP1:K ApoE chimeras. However, only the (GE )-(GD ) and (GE ) -(GD ) PATs yielded a 5-fold and a 4-fold, respectively, improvement in expression over the original TPP1-(GS) -K ApoE construct. Further investigation is required to understand the electrostatic and/or structural basis of these findings.