Release of DNA from Dermanyssus gallinae during the Biting Process

Simple Summary Like many hematophagous parasites, the poultry red mite Dermanyssus gallinae may release some material during the biting process. This investigation evidenced that small amounts of mite DNA may be found in chicken skin after D. gallinae infestation. Since the retrieved DNA is both of nuclear and mitochondrial origin, it is possible to hypothesize that, while biting, the mite releases cellular material. Dermanyssus gallinae is a hematophagous ectoparasitic mite that usually infests poultry, but is also known for occasionally attacking other animals and humans. It represents a major problem for poultry systems all over the world, with detrimental effects for both production and animal welfare. Despite the significance of D. gallinae, very little is known about the biting process to date. Therefore, this study has aimed to verify if mite DNA is injected into the host skin during the blood meal. Mite DNA has been detected by seminested PCR from infested chicken skin and quantified by real-time PCR. Furthermore, its localization within the host tissue has been checked by fluorescent in situ hybridization. Results showed that a very little amount of D. gallinae DNA can be released by mites, suggesting that the latter do not introduce whole or partially destroyed cells into the host, but rather it injects traces of nucleic acids, possibly together with merocrine secretions.