Morphological and molecular identification of multi-antibiotic resistant bacteria in the wound site of diabetic ulcers

Abstract. Risqiyah W, Narulita E, Rofiqoh A, Ludfi AS, Iqbal M. 2021. Morphological and molecular identification of multi-antibiotic resistant bacteria in the wound site of diabetic ulcers. Biodiversitas 23: 663-670. The study aimed to determine the characteristics of diverse species of bacteria found in diabetic ulcers and the inhibition of antibiotics against these bacteria. The method used is Gram staining for morphological characterization, identification using 16S rRNA gene, and pour plate for antibiotic resistance test. The morphological characterization result showed that the colonies had white color, circular shape, flat elevation, entire edge, small size, and basil shape with Gram negative type of bacteria. Another result showed yellow, greenish, pellucid, and green color, irregular shape, raised elevation, undulate and lobate edge, medium size, coccus and Gram positive type of bacteria. Query cover of molecular identification showed 73-100% and 77.61-96.77% for the result of similarity identification with Alcaligenes faecalis, Shigella flexneri, Enterococcus faecalis, Proteus mirabilis, and Acinetobacter seohaensis. The identification and antibiotic resistance testing showed that the bacterial species found in diabetic ulcers was A. faecalis strain NRBC 13111, which was resistant to all tested antibiotics, except A. faecalis strain NRBC 13111 from the UB 2.3K and A. faecalis strain NRBC 13111 from the sample UB 3.4M. Enterococcus faecalis strain ATCC 19433 was resistant to ceftriaxone, ceftazidime, clindamycin and metronidazole, intermediates to cefoperazone, and sensitive to ciprofloxacin. Proteus mirabilis strain JCM 1669 was resistant to clindamycin and metronidazole, sensitive to ceftriaxone and ciprofloxacin, intermediates to ceftazidime and cefoperazone. Proteus mirabilis strain ATCC 29906, A. seohaensis strain SW-100 and S. flexneri strain ATCC 29903 were resistant to all tested antibiotics.