Purification and characterisation of uricase from Bacillus subtilis SP6

Sneha O. Pustake,Prashant Bhagwat,Santhosh Pillai,Padma B. Dandge

Process Biochemistry（2022）

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摘要

•Bacillus subtilis SP6 was evaluated for its ability to produce uricase and allantoin.•Uricase was purified with 8.65-fold purification and 26.37 % yield and had a weight of 44 kDa.•Purified uricase comprises of 35.7 % α-helix, 41.8 % β-sheet and 3.8 % turns and Tm 42 °C.•Presence of allantoin was confirmed using LCMS.

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关键词

Uricase,Bacillus subtilis,Ion-exchange chromatography,Biophysical characterisation,Allantoin,Pharma-cosmeceutical industries

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