Quantification of chitosan in aqueous solutions by enzymatic hydrolysis and oligomer analysis via HPLC-ELSD

A new method for quantitative analysis of chitosan in aqueous solution is introduced, comprising an enzyme-driven cleavage to water-soluble chitooligosaccharides (COS), N-acetylation, separation via UHPLC and detection by use of an evaporative light scattering detector (ELSD). Chitosans with different fractions of acetylation (FA) and molecular weights (Mw) were hydrolyzed using a chitosanase/chitinase mixture. By subsequent N-acetylation with isotopically labelled acetic anhydride, COS mixtures with FA = 1 were obtained allowing for chromatographic separation solely based on their degree of polymerization (DP). ELSD data conversion into molar concentrations was realized using COS-specific external calibration curves, and mass spectrometry (MS) data informed about the chitosan's FA. The overall chitosan concentration was determined by simple addition of the COS concentrations multiplied by their DP. Validity of the method is shown for chitosan in presence of various co-solutes such as the protein BSA, the polysaccharide dextran and the monosaccharide glucosamine.