Evaluation Study on Extraction of Anthocyanins from Red Cabbage Using High Pressure CO2 + H2O: A Fuzzy Logic Model and Metabolomic Analysis

In this work, a fuzzy logic model was developed to elucidate the extraction performance of high-pressure CO2 + H2O compared with traditional H2O extraction and aqueous ethanol extraction. The high-pressure CO2 + H2O group acquired the highest comprehensive score considering yield, quality and stability. Both targeted and untargeted metabolomics results proved that the polarity of water was slightly modified; in particular, with the evidence from the untargeted metabolomics data, a higher proportion of water-insoluble compounds (2-methylindole, 3-formylindole, guanine, tyrosine and tryptophan) obtained by high-pressure CO2 + H2O extraction compared with traditional H2O extraction has been reported for the first time. Finally, the "3I" extraction mechanism of high-pressure CO2 + H2O is proposed, which offers an improvement in the solid-liquid mass transfer efficiency of phytochemicals, improving the polarity of solution and the isolation of O-2.