Comparison of Two RNA-Based Platforms for Detection of Fusions and Met Splicing Variant in Non Small Cell Lung Cancer Samples

ALK, ROS1, RET, NTRK fusions and MET exon 14 skipping variant (METex14) are present in 5-15% of advanced non-small cell lung cancer (NSCLC), and their identification is mandatory for selection of targeted therapies. Although FISH is considered the gold standard to determine gene fusions, their limitations are well known and it cannot be multiplexed. GeneReader Next Generation Sequencing (NGS) (Qiagen) and nCounter (Nanostring) allow multiple detection of fusion and splicing transcripts. The aim of the present work was to compare the two platforms in NSCLC samples.