Hydroxytyrosol Attenuates High-Fat-Diet-Induced Oxidative Stress, Apoptosis and Inflammation of Blunt Snout Bream (Megalobrama amblycephala) through Its Regulation of Mitochondrial Homeostasis

The present study was conducted to investigate the effects of dietary hydroxytyrosol (HT) on oxidative stress, inflammation and mitochondrial homeostasis in blunt snout bream (Megalobrama amblycephala). Fish were fed a low-fat diet (LFD, 5% lipid), a high-fat diet (HFD, 15% lipid), an LFD supplementing 200 mg/kg HT, or an HFD supplementing 200 mg/kg HT. After 10-week feeding, significant reduction of growth was observed in fish fed HFD, compared with other groups. HFD caused oxidative stress and more apoptosis of hepatocytes, while HT addition resulted in significant decrease of ROS and MDA contents, and the apoptotic hepatocytes. Moreover, the expression of genes involving inflammation of HFD group were elevated. Supplementing HT to HFD can attenuate this. All the activities of complexes of mitochondria in the HFD group were decreased compared with those in the LFD group, while supplementing HT to HFD significantly increased complex I-III activities. Furthermore, HFD downregulated the expressions of Atg5 and NRF-1 which induced the failure of mitophagy and biogenesis, while, supplementing HT to HFD reversed these expressions involving mitochondrial autophagy and biogenesis. In summary, adding HT to HFD relieved oxidative stress, apoptosis and inflammation, likely due to its regulation of mitochondrial homeostasis.