HRM method for identification of TP53 exon 5 and 8 mutations in human prostate cancer patients

Cagdas Gokhun Ozmerdiven,Ebubekir Dirican, Semih Ayan,Zeynep Tatar,Sami Cakir,Yavuz Guler,Abdullah Karadag,Tugba Soydas,Sevgi Karabulut Uzuncakmak, Melek Aru,Gonul Kanigur,Ahmet Ilvan

Meta Gene（2022）

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摘要

•The TP53 mutant is one of the most common mutant genes in PC.•HRM define variations in DNA sequence by measuring changes in fluorescence level of the melting DNA amplicon.•Mutations in TP53 exons 5 and 8 were observed in 12 (24%) patients by the Sanger method.•The detection sensitivity of the HRM method in exon 5 and exon 8 was 66.7% and 50%, respectively.

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关键词

HRM,Prostate cancer,Sanger,FFPE,TP53,PCR

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