Phosphoproteomics and Organelle Proteomics in Pancreatic Islets.

Özüm Sehnaz Caliskan,Giorgia Massacci,Natalie Krahmer,Francesca Sacco

Methods in molecular biology (Clifton, N.J.)（2022）

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摘要

Over the recent years, mass spectrometry (MS)-based proteomics has undergone dramatic advances in sample preparation, instrumentation, and computational methods. Here, we describe in detail, how a workflow quantifies global protein phosphorylation in pancreatic islets and characterizes intracellular organelle composition on protein level by MS-based proteomics.

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关键词

Mass spectrometry-based phosphoproteomics,Organelles,Pancreatic islets,Protein correlation profiling

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