Commercial ChIP-Seq Library Preparation Kits Performed Differently for Different Classes of Protein Targets.
Journal of biomolecular techniques : JBT(2022)
摘要
Our results suggest that the NEB protocol may be better for preparing H3K4me3 (and potentially other histone modifications with sharp peak enrichment) libraries; the Bioo protocol may be better for preparing H3K27me3 (and potentially other histone modifications with broad domain enrichment) libraries, and the Diagenode protocol may be better for preparing CTCF (and potentially other transcription factors with well-defined binding motifs) libraries. For ChIP-Seq experiments using novel targets without a known signal enrichment pattern, the NEB protocol might be the best choice, as it performed well for each of the 3 targets we tested across a wide array of input DNA levels.
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关键词
ChIP-Seq,library preparation,next generation sequencing,quality control
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