MP08-04 PHENOTYPIC CHARACTERIZATION OF A Mas1 RECEPTOR WILDTYPE AND KNOCKOUT ON BLADDER PAIN IN RATS

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology (MP08)1 Sep 2021MP08-04 PHENOTYPIC CHARACTERIZATION OF A Mas1 RECEPTOR WILDTYPE AND KNOCKOUT ON BLADDER PAIN IN RATS Maia Terashvili, Bidyut Medda, Bhavana Talluri, Banani Banerjee, and Jyoti Sengupta Maia TerashviliMaia Terashvili More articles by this author , Bidyut MeddaBidyut Medda More articles by this author , Bhavana TalluriBhavana Talluri More articles by this author , Banani BanerjeeBanani Banerjee More articles by this author , and Jyoti SenguptaJyoti Sengupta More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000001981.04AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The Angiotensin is widely known for its roles in blood pressure and fluid homeostasis. Recently it has been implicated in several facets of metastatic bone disease including inflammation, angiogenesis, tumor cell proliferation, and migration. In addition, in a model of prostaglandin-induced hyperalgesia, Ang-(1-7) dose-dependently attenuated behavioral signs of pain. Aim: The study was undertaken to test the hypothesis that Mas receptor activation by specific agonist could be an effective therapeutic target to alleviate bladder pain syndrome and/or chronic pelvic pain. METHODS: Two groups of female rats: Mas receptor wild type (Mas R WT) and knockout (KO) were used in this study. The electromyographic (EMG) recording from the external oblique muscles (EOM) of the abdomen during urinary bladder distension (UBD) is a direct method for measuring pain sensation in awake rats. In these experiments, rats were anesthetized with 2% Isoflurane and a pair of Teflon-coated electrodes were chronically implanted into the EOM for EMG recordings. Three days after the surgery, before EMG recording of viscero-motor reflex (VMR) to UBD rats were lightly anesthetized with isoflurane and a polyethylene catheter (PE-50) was inserted transurethrally into the bladder and sealed with tissue glue to prevent voiding. This allowed us to generate intravesical pressure without spontaneous voiding, a model of obstruction pain. The bladder was distended with saline either by slow infusion (0.1ml/min) or graded phasic isobaric distensions (10, 20, 30, 40 and 60mmHg). The increasing EOM contractions to incrementing intravesical pressures was considered as cramping pain of the abdomen represented as VMR. RESULTS: The Mas R WT rats exhibited a threshold for VMR at distending pressure >20mmHg, whereas Mas R KO exhibits threshold <10mmHg and higher. The results suggest that Mas R KO rats are more sensitive to visceral sensation compared to WT rats (FDistension (5, 66) = 132.3, p <0.0001). Mas R WT and KO rats injected with AVE0991, a Mas R agonist, abolished VMR to UBD in WT, but not in KO rats (FDistension (5, 66) = 132.3, p <0.0001). CONCLUSIONS: Based on these data Mas R may play a role in visceral pain perception. The Ang (1-7)/Mas receptor axis could be an effective therapeutic target to alleviate visceral pain. Source of Funding: This work was supported by NIH 2 R01 DK099201-05 and partly by Digestive Disease Center funds to J.N. Sengupta © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e151-e151 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Maia Terashvili More articles by this author Bidyut Medda More articles by this author Bhavana Talluri More articles by this author Banani Banerjee More articles by this author Jyoti Sengupta More articles by this author Expand All Advertisement Loading ...