Shish-kebab Structured Poly(ε-Caprolactone) Nanofibers Induce Bionic Mineralized Calcium Phosphate Coating for Bone Tissue Engineering

Mineralization is one of the important indexes to evaluate bone repair performance of bone repair materials. The rapid deposition and uniform distribution of mineral coating have always been a research focus of biomineralized membrane materials. Here, poly (ε-caprolactone) (PCL) nanofibers with shish-kebab (SK) structure biomimetic the surface features of collagen fibrils in bone tissue were created by electrospinning technology and self-induced crystallization methods. Biomimetic mineralization method was used to fabricate mineralized PCL nanofibrous membranes. SK structure increased the specific surface area of PCL nanofibrous membranes and provided more nucleation sites to promote mineralization and crystallization of calcium phosphate (CaP) mineralized layer, and mineralized PCL nanofibrous membrane significantly enhanced cell proliferation, attachment and ALP activity. SK structure and CaP coating synergistically promoted cell proliferation and osteoinductive ability of PCL nanofibrous membrane which had potential application value in the field of bone repair materials.