Camp−epac−pkcε−rim1α Signaling Regulates Presynaptic Long-Term Potentiation and Motor Learning
eLife(2023)
摘要
The cerebellum is involved in learning of fine motor skills, yet whether presynaptic plasticity contributes to such learning remains elusive. Here, we report that the EPAC-PKCe module has a critical role in a presynaptic form of long-term potentiation in the cerebellum and motor behavior in mice. Presynaptic cAMP-EPAC-PKCe signaling cascade induces a previously unidentified threonine phosphorylation of RIM1a, and thereby initiates the assembly of the Rab3A-RIM1a-Munc13-1 tripartite complex that facilitates docking and release of synaptic vesicles. Granule cell-specific blocking of EPAC-PKCe signaling abolishes presynaptic long-term potentiation at the parallel fiber to Purkinje cell synapses and impairs basic performance and learning of cerebellar motor behavior. These results unveil a functional relevance of presynaptic plasticity that is regulated through a novel signaling cascade, thereby enriching the spectrum of cerebellar learning mechanisms.
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关键词
cerebellum,plasticity,Purkinje cell,protein kinase A,Mouse
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