Fluorescence lifetime phantoms based on quenched coumarin derivatives

Christian Freymuller,Sviatlana Kalinina,Angelika Ruck,Ronald Sroka,Adrian Ruhm

Advances in Microscopic Imaging III（2021）

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摘要

Liquid reference samples for 2-photon fluorescence lifetime investigations were developed, based on coumarin 1 and 6 and 4-hydroxy-TEMPO as quencher. Spectral and fluorescence lifetime characteristics similar to intracellular NADH and FAD were achieved.

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