Fast cardiac imaging in live embryos using multiphoton light-sheet microscopy at low laser repetition rate

Vincent Maioli,Antoine Boniface,Pierre Mahou,Júlia Ferrer Ortas,Lamiae Abdeladim,Emmanuel Beaurepaire,Willy Supatto

Advances in Microscopic Imaging III（2021）

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摘要

We propose a framework to quantify photodamage in multiphoton light-sheet microscopy. Using cardiac imaging in live zebrafish embryos, we demonstrate an order of magnitude signal enhancement is safely obtained by adjusting the laser repetition rate.

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